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EVALUATION OF BACTERICIDAL ACTIVITY OF AN ANTISEPTIC GEL THROUGH THE DILUTION -NEUTRALIZATION METHOD

By: Arias-Palacios, Janeth Del Carmen.
Contributor(s): Esquivel, Libardo Hernandez.
Publisher: M P Innovare Academic Sciences Pvt Ltd 2019Edition: Vol.11(2).Description: 17-20p.Subject(s): PHARMACEUTICSOnline resources: Click here In: International journal of pharmacy and pharmaceutical scienceSummary: Objective: To evaluate the bactericidal activity of an antiseptic gel for hygiene and disinfection of the hands by rubbing. Method s: The dilution -neutralization method was used according to Colombian Technical Standard NTC, 2009, 12 , 16 in Instituto Colombiano de Normas Técnicas y Certificación ( ICONTEC), 5 strains, over 6 different types and with 6 replicas per time were exposed to the antiseptic gel, using Letheen Broth as a neutralizer substance. Result s: A 99% of reduction was obtained with Staphylococcus aureus 6538, Enterococos hirae 10541, Pseudomonas aer uginosa 15442 and Klebsiella sp , within the first 30 s of exposure to the gel and within the first 60 s of exposure using the Escherichia coli 19538. All strains used coming from American Type Culture Collection (ATCC) . Conclusio n: It was confirmed that the product is effective. In presence of a neutralizer substance, the microorganisms were not inhibit , so the growing reduction occurs due to the action of the product.
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Objective:
To
evaluate the bactericidal activity of an antiseptic gel for hygiene and disinfection of the hands
by rubbing.
Method
s:
The
dilution
-neutralization
method
was used
according to Colombian Technical Standard NTC, 2009, 12
, 16
in Instituto Colombiano de
Normas Técnicas y Certificación (
ICONTEC),
5 strains, over 6 different types and with 6 replicas per time were
exposed to the antiseptic gel, using
Letheen Broth as a neutralizer substance.
Result
s:
A 99% of reduction was obtained with
Staphylococcus aureus
6538,
Enterococos
hirae
10541,
Pseudomonas aer
uginosa
15442 and
Klebsiella
sp
, within the first 30 s of exposure to the gel and within the first 60 s of exposure using the
Escherichia
coli 19538.
All strains used
coming
from
American
Type Culture Collection (ATCC)
.
Conclusio
n:
It was confirmed that the product is effective. In presence of a neutralizer
substance, the microorganisms
were not inhibit
, so the
growing
reduction occurs due to the action of the product.

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